Proximity Extension Assay (PEA) technology

Innovation for successful protein biomarker discovery

The biomarker panels developed by Olink is a unique technology enabling high-throughput, multiplex immunoassays that measure 92 proteins across 96 samples simultaneously using only one microliter of serum, plasma, or almost any other type of biological sample. The innovative dual recognition, DNA-coupled methodology provides exceptional readout specificity, enabling high multiplex, rapid throughput biomarker analysis without compromising on data quality assay robustness.

The 981 immuno assays developed are divided into 12 disease focused panels. Samples may be analyzed against one or more panels using only 1µl of plasma/serum per panel.

The content of each panel can be found at

Olink assay overview

The main steps involved in an assay are outlined below. A pair of oligonucleotide-labeled antibodies, Proseek probes, are allowed to pair-wise bind to the target protein present in the sample in a homogeneous assay, with no need for washing. When the two Proseek probes are in close proximity, a new PCR target sequence is formed by a proximity-dependent DNA polymerization event. The resulting sequence is subsequently detected and quantified using Fluidigm Biomark HD real-time PCR system that allow the analysis of 96 samples against 96 assays in nanoliter scale within 3 hours.

More info on PEA

More info